ELISA Pilot Dilution Planner

Coverage, not validationShows what each dilution can mathematically cover.

Local and privateInputs stay in this browser page.

Direct-prep checkFlags volumes below your reliable pipetting minimum.

Planning method

Choose how factors are generated

Auto coverageLog-centered factors Fixed-foldYour own series

Concentration ranges

Define the interval to cover

Usable assay range Required

Enter the interval your kit, validated method, or lab-defined procedure says you can use for quantitative interpretation. It may be narrower than the full standard or detection range.

Expected original target-analyte concentration Rough estimate

I only know an upper bound

Use a broad range if uncertain. Do not substitute total protein concentration unless it truly represents the target analyte.

Pilot budget

Set wells and preparation limits

Pilot dilution points 1–12 distinct concentrations. Sample volume per well

µL

Sample or diluted sample added per well. Replicates per point Duplicates = 2; triplicates = 3. Preparation overage

Extra volume for pipetting loss. Minimum reliable pipetting volume

µL

Below this, the result asks for an intermediate dilution instead of presenting direct prep as reliable.